Evaluation of the Value of Quantitative Assay of Hepatitis B Virus Covalently Closed Circular DNA in Diagnosis and Therapy of Chronic Hepatitis B 慢性乙型肝炎共价闭合环状DNA定量测定在诊断和治疗中的价值
Objective To study the effect of hepatitis B virus ( HBV) C protein C-terminal small interference RNA ( siRNA) on reduction of HBV covalently closed circular DNA ( cccDNA) level. 目的研究乙型肝炎病毒(HBV)C蛋白羧基端短小的干扰RNA(siRNA)对HBV超螺旋双链闭环DNA(cccDNA)水平的影响。
The pBR322 recombinant plasmids DNA products, which purified by phenol-extract and equilibrium centrifugation in CsCl-ethidium bromide gradients, have been shown as mainly spatial conformation of covalently closed circular DNA ( i. e. cccDNA) by agarose gel electrophoresis for homogeneity detection. 将pBR322重组质粒DNA纯化,经琼脂糖凝胶电泳鉴定其主要具共价闭合环状空间构象。
Once HBV has entered the host cell, its genome is converted into a covalently closed circular DNA ( CCC-DNA). 当HBV进入宿主细胞后,基因组转变为共价闭合环状DNA(CCC-DNA)。
The genome is a double stranded, covalently closed, circular, supercoiled DNA molecule, approximately 8000 base pairs in size. 基因组为闭合环状双链超螺旋DNA,由大约8000对碱基组成;
A Simple Method for Isolation of Covalently Closed Circular DNA Molecules 一种简易的抽提共价闭合环状DNA的方法
[ Objective] To study the clinical significance of serum hepatitis B virus covalently closed circular DNA ( HBV cccDNA) determination in patients with chronic HBV infection. 探讨慢性乙型肝炎病毒感染者外周血乙型肝炎病毒共价闭合环状DNA检测的临床意义。
In addition, the current antiviral therapy can not completely remove covalently closed circular DNA ( cccDNA), and can not completely clear the hepatitis B virus. 而且,现在的抗病毒疗法都不能彻底清除共价闭合环状DNA(cccDNA),不能彻底的清除乙肝病毒。